Miyu is a young vampire girl. She was 13 when time for her was stopped, and she was given the duty of hunting Shinma. Yet Miyu isn't an ordinary vampire. She is unaffected by sunlight, crosses, and holy water. She has within her, the power to kill, or bestow life everlasting. Miyu needs blood of humans to exist, but shows a preference for beautiful people. Miyu has a very distinct haunting laugh of an eternally young girl, even though she endures everlasting sorrow within her.

Larva is a rather ominous figure who lurks in the background. Larva is a western Shinma, who crossed the ocean to kill Miyu (before her vampire blood awoke), and rule the Shinma in Japan. The plan backfired however, and Miyu drank his blood, and bound him to her. Larva serves as her willing companion, friend, and servant. He wears a mask to hide his shame, and in the OAV never speaks. Although in the manga he is often speaks without a mask. Larva is the only one who understands Miyu's sadness.

Himiko makes her living as a spiritualist (and not with the psychic hotline either). She uses her abilities to investigate instances where no one else can help. She is a very logical, levelheaded person, and despises Miyu because she's a vampire. Eventually she begins to seek out Miyu in order to find answers, although some of the answers she gets, she will not like...

Yui is a young girl is part Shinma, part vampire, and part Shi (another sort of supernatural being). Yui also has a duty, which is to awaken her sister Yuki who is in eternal asleep (who's supposed to be protecting a magical lake). Yui however is not strong enough to do this (yet), but as fortunately a male Shi named Nagi comes to protect her. Yui is much more ... human in character, and emotion than Miyu. Yui is not the predator that Miyu is, in fact Yui is often the intended victim. She has her own series, makes appearances in Vampire Miyu.

Very energetic and full of life, Chisato is a very vibrant cheerful girl. While you would think her personality would be completely at odds with that of serious and stoic Miyu, yet her charm wins even Miyu over. Chisato and Miyu become friends rather quickly even though Chisato finds Miyu to be a tad bit strange at times. One rather interesting casual remark she makes to Miyu is: "You talk like my grandmother sometimes..."

Yukari is sited to be a very strong girl, both physically and in will. She's a tad bit rough around the edges, and occasionally too blunt, but still a caring person. While the typical anime formula sites that she wouldn't be all that smart, this in fact is not the case (she's average in the intelligence department). Yukari was already friends with Chisato, and Hisae before Miyu arrived. She is not sure what to think of Miyu.

Hisae is the brains of the bunch you could say (where as Chisato is the motivation, and Yukari the brawn). Hisae is said to be a bookworm, and she looks the part (so of course she has glasses). She is well spoken, and very analytical. If anyone would know anything about a subject, it would probably be Hisae.

Reiha is a snow demon who has been acquainted with Miyu for some time. Although she is always very polite to Miyu, the two are at odds with each other. Reiha would also like to do away with stray shinma (because they make real super natural beings look bad). Where as Miyu has sympathy and compassion for humans (at times), Reiha has none, and does not value human life. She carries around a very opinionated doll as her companion.

Shiina is a minor shinma, who along with Larva is a companion of Miyu. Shiina however is the newcomer on the scene, for which Miyu often teases it. While not having any real fighting power, Shiina can see through any guise using the eye which is often hidden by it's ear. It can also often break certain powers of other shinma. Shiina has a very 'down to earth' view of the world.